Spag16, an Axonemal Central Apparatus Gene, Encodes a Male Germ Cell Nuclear Speckle Protein that Regulates SPAG16 mRNA Expression
2011
SPAG16: A Gene that Encodes Two Proteins Important for Male Fertility
publication
Evidence: moderate
Author Information
Author(s): Nagarkatti-Gude David R., Jaimez Ruth, Henderson Scott C., Teves Maria E., Zhang Zhibing, Strauss Jerome F. III
Primary Institution: Virginia Commonwealth University
Hypothesis
Does SPAG16S play a role in regulating SPAG16L expression in male germ cells?
Conclusion
SPAG16S is crucial for the expression of SPAG16L and plays a significant role in spermatogenesis.
Supporting Evidence
- SPAG16S is expressed only in male germ cells and is crucial for spermatogenesis.
- Transduction of SPAG16S into cultured cells increases SPAG16L expression.
- SPAG16S localizes to nuclear speckles, suggesting a role in gene regulation.
Takeaway
There are two proteins made from the SPAG16 gene, and one of them helps the other one work better in male reproductive cells.
Methodology
The study involved gene expression analysis, immunohistochemistry, and transduction experiments in cultured cells.
Statistical Information
P-Value
p<0.05
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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