In vivo changes in zebrafish anesthetic sensitivity in response to the loss of kif5Aa are associated with the alteration of mitochondrial motility
2024
Zebrafish Anesthetic Sensitivity and Mitochondrial Motility Changes
publication
Author Information
Author(s): Bedell Victoria, Dubey Priya, Datta Roshni, Eckenhoff Roderic G
Primary Institution: Cold Spring Harbor Laboratory
Hypothesis
The loss of kif5Aa alters anesthetic sensitivity and mitochondrial motility in zebrafish.
Conclusion
The loss of kif5Aa increases sensitivity to certain anesthetics while affecting mitochondrial motility differently.
Supporting Evidence
- The loss of kif5Aa increases behavioral sensitivity to propofol and etomidate.
- Kif5Aa KO animals were resistant to the behavioral effects of dexmedetomidine.
- Propofol inhibited mitochondrial motility in WT zebrafish neurons.
- Etomidate inhibited mitochondrial motility in both anterograde and retrograde directions.
Takeaway
When zebrafish lose a specific protein, they react more strongly to some sleep drugs, and the way their tiny energy factories move changes too.
Methodology
Zebrafish behavioral assays and primary neuronal cell culture were used to assess anesthetic sensitivity and mitochondrial motility.
Digital Object Identifier (DOI)
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