Identifying Genes in Wheat Stripe Rust Interaction
Author Information
Author(s): Ma Jinbiao, Huang Xueling, Wang Xiaojie, Chen Xianming, Qu Zhipeng, Huang Lili, Kang Zhensheng
Primary Institution: Northwest A&F University
Hypothesis
The study aims to develop an EST database of wheat infected by Puccinia striiformis to determine transcription profiles of genes involved in compatible wheat-Pst interaction.
Conclusion
The cDNA library is useful for identifying functional genes involved in the wheat-Pst compatible interaction and has established a new database for studying Pst pathogenesis genes and wheat defense genes.
Supporting Evidence
- 5,793 ESTs were obtained and clustered into 2,743 unique sequences.
- 52.8% of the ESTs were homologous to plant genes.
- Quantitative real-time PCR confirmed differential expression of seven genes in compatible and incompatible interactions.
Takeaway
Researchers created a library of genes from wheat infected by a fungus to understand how the fungus interacts with the plant without causing immediate harm.
Methodology
Total RNA was extracted from infected wheat leaves, and a cDNA library was constructed and analyzed for gene expression.
Digital Object Identifier (DOI)
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