Study of Listeria monocytogenes σB regulon
Author Information
Author(s): Hain Torsten, Hossain Hamid, Chatterjee Som S, Machata Silke, Volk Ute, Wagner Sandra, Brors Benedikt, Haas Stefan, Kuenne Carsten T, Billion Andre, Otten Sonja, Pane-Farre Jan, Engelmann Susanne, Chakraborty Trinad
Primary Institution: Institute for Medical Microbiology, Justus-Liebig-University, Giessen, Germany
Hypothesis
The study investigates the role of the σB regulon in the stress response of Listeria monocytogenes.
Conclusion
The σB regulon in Listeria monocytogenes includes genes that help the bacterium survive in stressful environments.
Supporting Evidence
- The study identified 216 σB-dependent genes in L. monocytogenes.
- 105 genes were positively regulated by σB, while 111 were negatively regulated.
- The σB regulon includes genes involved in stress response, metabolism, and virulence.
Takeaway
This study looks at how a specific part of the bacteria helps it survive tough conditions, like heat and acid.
Methodology
The study used quantitative real-time PCR and immuno-blot analysis to examine σB expression and whole genome-based transcriptional profiling to identify σB-dependent genes.
Limitations
The study only monitored transcriptional activity under specific growth conditions in complex media.
Digital Object Identifier (DOI)
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