Tagenerator: A Tool for Generating RNA-Specific PCR Tags
Author Information
Author(s): Fernando Lopes Pinto, Håkan Svensson, Peter Lindblad
Primary Institution: Uppsala University
Hypothesis
Can a software tool be developed to generate non-genomic oligonucleotide tag sequences for RNA template-specific PCR?
Conclusion
Tagenerator can generate tag sequences that avoid genomic DNA contamination while maintaining good priming properties for RT-PCR experiments.
Supporting Evidence
- Tagenerator generates tags that do not amplify genomic DNA.
- Tags specifically amplify cDNA, allowing for accurate RNA testing.
- The software has been used in various molecular experiments.
Takeaway
Tagenerator is a computer program that helps scientists create special tags for testing RNA without mixing in DNA, making their results more accurate.
Methodology
The program was developed in Perl and tested through molecular experiments to evaluate the performance of the generated tags.
Limitations
The software may not always provide the most suitable tag for every experiment due to variations in melting temperatures and potential secondary structures.
Digital Object Identifier (DOI)
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