WSSV449 Activates NF-κB Pathway for Gene Expression
Author Information
Author(s): Wang Pei-Hui, Gu Zhi-Hua, Wan Ding-Hui, Zhang Ming-Yan, Weng Shao-Ping, Yu Xiao-Qiang, He Jian-Guo
Primary Institution: MOE Key Laboratory of Aquatic Product Safety / State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-Sen University, Guangzhou, People’s Republic of China
Hypothesis
WSSV449 activates the Toll-mediated NF-κB pathway for regulating viral gene expression.
Conclusion
WSSV449 activates the NF-κB pathway to facilitate the expression of specific viral genes in shrimp.
Supporting Evidence
- WSSV449 shows similarity to host Tube, a positive regulator of the Toll pathway.
- WSSV449 can activate the promoters of both shrimp and Drosophila antimicrobial peptide genes.
- Overexpression of WSSV449 induces the promoter activities of WSSV069 (ie1), WSSV303, and WSSV371.
Takeaway
A virus called WSSV can trick shrimp's immune system to help it make more copies of itself by turning on a special pathway in the shrimp's cells.
Methodology
The study involved genome-wide analysis, promoter screening, and dual luciferase reporter assays in Drosophila S2 cells.
Limitations
The study primarily focuses on in vitro experiments, which may not fully represent in vivo conditions.
Statistical Information
P-Value
p<0.01
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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