American Mink BAC Library and Gene Sequencing
Author Information
Author(s): Anistoroaei Razvan, Hallers Boudewijn, Nefedov Michael, Christensen Knud, de Jong Pieter
Primary Institution: University of Copenhagen
Hypothesis
The study aims to construct a BAC library for the American mink and identify candidate genes important for the fur industry.
Conclusion
The CHORI-231 American mink BAC library will facilitate the identification of genes and genomic regions relevant to the fur industry.
Supporting Evidence
- The BAC library contains approximately 165,888 clones with an average insert size of 170 kb.
- Nineteen out of twenty-one candidate genes were successfully identified through screening.
- The library provides a 10-fold coverage of the mink genome based on estimated genome size.
Takeaway
Researchers created a library of mink DNA to help find genes that affect fur traits, which can improve breeding.
Methodology
The study involved constructing a BAC library from American mink DNA and sequencing candidate genes using 454 Roche technology.
Limitations
The study does not specify the exact genome size of the American mink, which may affect the interpretation of the BAC library's coverage.
Digital Object Identifier (DOI)
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