Evaluation of light microscopy and rapid diagnostic test for the detection of malaria under operational field conditions: a household survey in Ethiopia
2008

Evaluating Malaria Detection Methods in Ethiopia

Sample size: 11504 publication 10 minutes Evidence: moderate

Author Information

Author(s): Endeshaw Tekola, Gebre Teshome, Ngondi Jeremiah, Graves Patricia M, Shargie Estifanos B, Ejigsemahu Yeshewamebrat, Ayele Berhan, Yohannes Gedeon, Teferi Tesfaye, Messele Ayenew, Zerihun Mulat, Genet Asrat, Mosher Aryc W, Emerson Paul M, Richards Frank O

Primary Institution: The Carter Center, Addis Ababa, Ethiopia

Hypothesis

What is the agreement between light microscopy and rapid diagnostic tests (RDT) for malaria detection in Ethiopia?

Conclusion

Blood slide microscopy is the preferred method for detecting malaria parasites in population-based surveys.

Supporting Evidence

  • The overall slide positivity rate was 4.1%.
  • ParaScreen RDT was positive in 3.3% of those tested.
  • ParaScreen RDT exhibited high specificity of 98.5%.
  • The overall sensitivity of ParaScreen RDT was 47.5%.

Takeaway

This study looked at how well two tests for malaria work in Ethiopia. It found that the traditional blood slide test is better than the quick test.

Methodology

A population-based survey was conducted in 224 randomly selected clusters of 25 households each, testing blood samples using microscopy and RDT.

Potential Biases

Potential bias due to the handling and storage conditions of RDTs.

Limitations

The study did not quantify parasite density and did not record clinical history of participants.

Participant Demographics

53.8% of participants were female, with a mean age of 20.7 years.

Statistical Information

P-Value

0.001

Confidence Interval

95% CI 3.4–5.0%

Statistical Significance

p<0.05

Digital Object Identifier (DOI)

10.1186/1475-2875-7-118

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