Complete chloroplast genome of Oncidium Gower Ramsey
Author Information
Author(s): Wu Fu-Hui, Chan Ming-Tsair, Liao De-Chih, Hsu Chen-Tran, Lee Yi-Wei, Daniell Henry, Duvall Melvin R, Lin Choun-Sea
Primary Institution: Agricultural Biotechnology Research Center, Academia Sinica, Taipei, Taiwan
Hypothesis
Molecular markers derived from the chloroplast genome can provide useful tools for phylogenetic resolution in Oncidiinae.
Conclusion
The complete chloroplast genome information is useful for plant phylogenetic and evolutionary studies in Oncidium with applications for breeding and variety identification.
Supporting Evidence
- The Onc. Gower Ramsey chloroplast genome is 146,484 bp long.
- Most ndh genes in Oncidiinae are non-functional, except for ndhE.
- Phylogenetic analysis identified relationships among 15 Oncidiinae hybrids.
Takeaway
Scientists studied the DNA of a type of orchid to help figure out how different types are related and how to breed them better.
Methodology
The complete chloroplast genome was sequenced using PCR and Sanger sequencing, and phylogenetic analysis was conducted on 15 Oncidiinae varieties.
Limitations
The study is limited by the lack of complete chloroplast genome sequences for other orchids, which may affect the phylogenetic analysis.
Participant Demographics
Fifteen commercial Oncidiinae varieties were obtained from a grower in Taiwan.
Digital Object Identifier (DOI)
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