Multi-Locus Variable-Number Tandem Repeat Profiling of Salmonella enterica Serovar Typhi Isolates from Blood Cultures and Gallbladder Specimens from Makassar, South-Sulawesi, Indonesia
2011

Genetic Diversity of Salmonella Typhi in Makassar, Indonesia

Sample size: 297 publication 10 minutes Evidence: high

Author Information

Author(s): Hatta Mochammad, Pastoor Rob, Scheelbeek Pauline F. D., Sultan Andi R., Dwiyanti Ressy, Labeda Ibrahim, Smits Henk L.

Primary Institution: Hasanuddin University, Makassar, South-Sulawesi, Indonesia

Hypothesis

What is the genetic diversity of Salmonella enterica serovar Typhi in blood cultures and gallbladder specimens from patients in Makassar, Indonesia?

Conclusion

The study found a large diversity of Salmonella Typhi genotypes in blood cultures and identified a unique genotype in gallbladder specimens from cholelithiasis patients.

Supporting Evidence

  • 297 Salmonella Typhi blood culture isolates were analyzed, revealing 76 distinct genotypes.
  • Four unique S. Typhi isolates were found in gallbladder specimens from cholelithiasis patients.
  • The majority of culture-positive patients had moderate disease conditions.
  • Genotype EInd100 was not found in any blood culture isolates from typhoid patients.

Takeaway

Scientists studied bacteria that cause typhoid fever in Indonesia and found many different types, including one special type from patients with gallbladder issues.

Methodology

Multi-locus variable-number tandem repeat analysis was used to genotype Salmonella Typhi isolates from blood cultures and gallbladder specimens.

Limitations

The study did not assess the precise morphological location of infections in gallbladder patients and relied on culture methods that may miss some infections.

Participant Demographics

The study included 297 typhoid patients with a median age of 29 years, and four cholelithiasis patients aged 24 to 42 years.

Statistical Information

Confidence Interval

0.783 to 0.910

Digital Object Identifier (DOI)

10.1371/journal.pone.0024983

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