Heterofucan from Sargassum filipendula Induces Apoptosis in HeLa Cells
Author Information
Author(s): Costa Leandro Silva, Telles Cinthia Beatrice Silva, Oliveira Ruth Medeiros, Nobre Leonardo Thiago Duarte Barreto, Dantas-Santos Nednaldo, Camara Rafael Barros Gomes, Costa Mariana Santana Santos Pereira, Almeida-Lima Jailma, Melo-Silveira Raniere Fagundes, Albuquerque Ivan Rui Lopes, Leite Edda Lisboa, Rocha Hugo Alexandre Oliveira
Primary Institution: Laboratory of Biotechnology of Natural Polymers (BIOPOL), Departament of Biochemistry, Federal University of Rio Grande do Norte (UFRN), Natal-RN, Brazil
Hypothesis
The primary objective of this study was to determine the relevant mechanisms for an antiproliferative effect of the heterofucan SF-1.5v.
Conclusion
Heterofucan SF-1.5v induces apoptosis in HeLa cells mainly by releasing apoptosis-inducing factor (AIF) from mitochondria into the cytosol.
Supporting Evidence
- SF-1.5v showed antiproliferative activity on HeLa cells.
- SF-1.5v induced apoptosis without activating caspases.
- SF-1.5v decreased Bcl-2 expression and increased Bax expression.
Takeaway
A substance from seaweed can help kill cancer cells by making them self-destruct in a special way that doesn't involve the usual cell death processes.
Methodology
The study involved extracting heterofucan SF-1.5v from Sargassum filipendula and testing its effects on HeLa cells through various assays including MTT and annexin V/PI staining.
Limitations
The study does not explore the effects of SF-1.5v on other cancer cell lines or its potential side effects.
Statistical Information
P-Value
p<0.05
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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