Identification of two regulatory binding sites which confer myotube specific expression of the mono-ADP-ribosyltransferase ART1 gene
2008
Regulation of ART1 Gene Expression in Muscle Cells
publication
Evidence: moderate
Author Information
Author(s): Friedrich Maik, Böhlig Levin, Kirschner Ralf D, Engeland Kurt, Hauschildt Sunna
Primary Institution: University of Leipzig
Hypothesis
The study investigates the regulatory mechanisms of the ART1 gene expression during myogenesis.
Conclusion
The ART1 gene is regulated by myogenin and MEF-2 during muscle cell differentiation.
Supporting Evidence
- ART1 expression is restricted to myotube formation.
- Myogenin binds to the E box and MEF-2 binds to the A/T-rich element in the ART1 promoter.
- Mutating the E box or A/T-rich element significantly reduces ART1 promoter activity.
Takeaway
The ART1 gene helps muscle cells stick together, and it turns on when muscle cells grow and change into myotubes.
Methodology
The study used C2C12 and C3H-10T 1/2 cell lines to analyze the ART1 promoter and its regulatory elements through various molecular biology techniques.
Digital Object Identifier (DOI)
Want to read the original?
Access the complete publication on the publisher's website