Development of SSR markers for pearl millet
Author Information
Author(s): Senthilvel S, Jayashree B, Mahalakshmi V, Kumar P Sathish, Nakka S, Nepolean T, Hash CT
Primary Institution: International Crops Research Institute for the Semi-Arid Tropics (ICRISAT)
Hypothesis
The study aimed to develop additional SSR markers for the pearl millet research community.
Conclusion
The study successfully developed new EST-derived SSR markers that can be used for genetic improvement in pearl millet.
Supporting Evidence
- A total of 164 sequences containing SSRs were identified from the ESTs.
- Ninety primer pairs were designed, with 58 yielding clear amplification products.
- New markers were mapped to previously unfilled gaps in the pearl millet linkage maps.
- EST-SSRs were found to be more likely to function in distantly related species compared to genomic SSRs.
Takeaway
Researchers created new markers to help improve pearl millet, a vital crop for many people. These markers make it easier to study and breed better varieties of the plant.
Methodology
The study involved data mining of 3520 expressed sequence tags (ESTs) to identify and develop SSR markers.
Limitations
The study's findings may not fully represent the pearl millet genome due to the limited number of EST sequences analyzed.
Digital Object Identifier (DOI)
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