Activity In Vivo of Anti-Trypanosoma cruzi Compounds Selected from a High Throughput Screening
2011

Testing Anti-Trypanosoma cruzi Compounds for Chagas Disease

Sample size: 10 publication 10 minutes Evidence: moderate

Author Information

Author(s): Andriani Grasiella, Chessler Anne-Danielle C., Courtemanche Gilles, Burleigh Barbara A., Rodriguez Ana

Primary Institution: New York University School of Medicine

Hypothesis

Can high throughput screening identify effective compounds against Trypanosoma cruzi?

Conclusion

Two compounds significantly reduced T. cruzi infection in a mouse model after five days of treatment.

Supporting Evidence

  • 23 compounds were selected from high throughput screening for further analysis.
  • Half of the compounds confirmed anti-T. cruzi activity in vitro.
  • Two compounds reduced T. cruzi infection in mice by more than 90%.

Takeaway

Scientists are looking for new medicines to help treat Chagas disease, and they found two promising compounds that work really well in mice.

Methodology

The study involved high throughput screening to select compounds, followed by in vitro and in vivo testing in mice.

Limitations

Some compounds that showed activity in initial tests did not perform well in vivo, indicating a need for further validation.

Participant Demographics

Balb/c mice were used for in vivo testing.

Statistical Information

P-Value

p<0.05

Statistical Significance

p<0.05

Digital Object Identifier (DOI)

10.1371/journal.pntd.0001298

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