How Certain Breast Cancer Cells Resist Radiation
Author Information
Author(s): Yin Hong, Glass Jonathan, Muzi-Falconi Marco
Primary Institution: Feist-Weiller Cancer Center and Department of Medicine, Louisiana State University Health Sciences Center
Hypothesis
The study investigates whether the CD44+/CD24− or low breast cancer cells exhibit enhanced radiation resistance due to increased ATM signaling.
Conclusion
The CD44+/CD24− or low breast cancer cells show increased radiation resistance, which can be reduced by targeting ATM signaling.
Supporting Evidence
- The CD44+/CD24− or low subset showed increased sphere formation and resistance to radiation compared to the non-CD44+/CD24− or low subset.
- Activation of ATM signaling was significantly increased in CD44+/CD24− or low cells compared to non-CD44+/CD24− or low cells.
- Application of an ATM inhibitor effectively decreased the radiation resistance of the CD44+/CD24− or low subset.
Takeaway
Some breast cancer cells are like superheroes that can survive radiation better than others, but we found a way to make them weaker by blocking a special signal they use.
Methodology
The study involved isolating CD44+/CD24− or low breast cancer cells and comparing their radiation resistance to other cell subsets through clonogenic survival assays.
Potential Biases
Potential bias in selecting cell lines and patient samples may affect the generalizability of the findings.
Limitations
The study primarily focuses on in vitro experiments, which may not fully replicate in vivo conditions.
Participant Demographics
The study included breast cancer cell lines (MCF-7, MDA-MB-231) and primary cultures from two patients with breast cancer.
Statistical Information
P-Value
p<0.01
Statistical Significance
p<0.01
Digital Object Identifier (DOI)
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