Using NanoString Technology for mRNA Measurement in Archived Cancer Samples
Author Information
Author(s): Patricia P. Reis, Levi Waldron, Rashmi S. Goswami, Wei Xu, Yali Xuan, Bayardo Perez-Ordonez, Patrick Gullane, Jonathan Irish, Igor Jurisica, Suzanne Kamel-Reid
Primary Institution: University Health Network, Toronto, ON, Canada
Hypothesis
Can the NanoString nCounter™ technology accurately quantify mRNA transcripts in archived FFPE oral carcinoma samples compared to traditional RQ-PCR methods?
Conclusion
The NanoString method provides superior gene expression quantification results compared to RQ-PCR in archived FFPE samples.
Supporting Evidence
- NanoString technology showed a higher correlation coefficient of 0.90 between fresh-frozen and FFPE samples.
- RQ-PCR analysis revealed a lower correlation coefficient of 0.53 between fresh-frozen and FFPE samples.
- The study included a total of 38 tumor samples from 19 patients.
Takeaway
This study shows that a new technology called NanoString can measure gene levels in old cancer samples better than the traditional method.
Methodology
The study compared mRNA transcript levels in fresh-frozen and FFPE samples using NanoString and RQ-PCR methods.
Potential Biases
Potential bias due to the quality of RNA extracted from FFPE samples.
Limitations
The study is limited by the degradation of RNA in FFPE samples, which may affect results.
Participant Demographics
19 patients with oral squamous cell carcinoma.
Statistical Information
P-Value
<0.0001
Statistical Significance
p<0.0001
Digital Object Identifier (DOI)
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