Zinc Regulation of the Yeast Zap1 Transcription Factor
Author Information
Author(s): Avery G. Frey, Amanda J. Bird, Marguerite V. Evans-Galea, Elizabeth Blankman, Dennis R. Winge, David J. Eide
Primary Institution: University of Wisconsin-Madison
Hypothesis
How does zinc status regulate the DNA binding activity of the Zap1 transcription factor in yeast?
Conclusion
Zinc regulates Zap1 activity through multiple mechanisms, including direct control of its DNA binding activity.
Supporting Evidence
- Zap1 controls its own expression via positive autoregulation.
- Zinc binding to Zap1's activation domains regulates its function.
- Zinc levels affect Zap1 DNA binding without altering its nuclear localization.
Takeaway
Zinc helps control a protein called Zap1 in yeast, which is important for managing zinc levels in the cell.
Methodology
The study used in vivo dimethyl sulfate footprinting and chromatin immunoprecipitation to assess Zap1 DNA binding activity.
Limitations
The footprinting method does not definitively show that the protein protecting the ZRE is Zap1 and not some other transcription factor.
Statistical Information
P-Value
p<0.05
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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