H2-driven xylitol production in Cupriavidus necator H16
Author Information
Author(s): Tytti Jämsä, Nico J. Claassens, Laura Salusjärvi, Antti Nyyssölä
Primary Institution: VTT Technical Research Centre of Finland Ltd.
Hypothesis
Can Cupriavidus necator H16 be used as a whole-cell biocatalyst for xylitol production using hydrogen as the electron donor?
Conclusion
The study demonstrated that Cupriavidus necator H16 can efficiently convert d-xylose to xylitol using hydrogen for cofactor recycling.
Supporting Evidence
- Over 90% of the energy from hydrogen was utilized for the bioconversion.
- The highest xylitol productivity reached was 0.7 g/L/h.
- Nearly complete bioconversion of 30 g/L of d-xylose into xylitol was achieved.
Takeaway
This study shows that a special bacteria can turn sugar into a sweetener using hydrogen, which is a clean energy source.
Methodology
Resting cells of Cupriavidus necator H16 were used to convert d-xylose to xylitol with hydrogen as the electron donor.
Limitations
The study did not explore the effects of elevated pressure on hydrogen solubility, which could enhance conversion rates.
Digital Object Identifier (DOI)
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