Cytokine Profiles in Dengue Patients
Author Information
Author(s): Fernando A Bozza, Oswaldo G Cruz, Sonia MO Zagne, Elzinandes L Azeredo, Rita MR Nogueira, Edson F Assis, Patricia T Bozza, Claire F Kubelka
Primary Institution: Instituto de Pesquisa Clínica Evandro Chagas, Fundação Oswaldo Cruz, Rio de Janeiro, Brazil
Hypothesis
Can plasma cytokine concentrations help in stratifying the severity of dengue disease?
Conclusion
The study found that MIP-1β and IFN-γ are significant markers for predicting dengue severity.
Supporting Evidence
- IL-1β, IFN-γ, IL-4, IL-6, IL-13, IL-7, and GM-CSF were significantly increased in severe dengue patients.
- MIP-1β levels were higher in mild dengue cases.
- MIP-1β and IFN-γ were independently associated with dengue severity and disease outcome.
Takeaway
Doctors can use certain proteins in the blood to tell if a dengue patient is likely to get very sick or not.
Methodology
A 17-cytokine multiplex fluorescent microbead immunoassay was used to analyze plasma from dengue patients.
Potential Biases
Potential bias in disease severity classification due to varying criteria.
Limitations
The small sample size limits the generalizability of the results, and only one time point was used for cytokine measurement.
Participant Demographics
59 dengue patients (33 females, 26 males, ages 15-73) from three health centers in Brazil.
Statistical Information
P-Value
p<0.05
Confidence Interval
0.045–0.72 for MIP-1β; 1.0541–1.245 for IFN-γ
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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