MicroRNA-196a regulates bovine newborn ovary homeobox gene (NOBOX) expression during early embryogenesis
2011
How miR-196a Affects Bovine NOBOX During Early Embryo Development
publication
Evidence: moderate
Author Information
Author(s): Tripurani Swamy K, Lee Kyung-Bon, Wee Gabbine, Smith George W, Yao Jianbo
Primary Institution: West Virginia University
Hypothesis
NOBOX is targeted by microRNAs for silencing and/or degradation during early embryogenesis.
Conclusion
miR-196a is a negative regulator of NOBOX during bovine early embryogenesis.
Supporting Evidence
- miR-196a was found to be expressed in both oocytes and embryos.
- Ectopic expression of miR-196a reduced NOBOX protein levels in HeLa cells.
- Microinjection of miR-196a mimic into bovine embryos significantly decreased NOBOX mRNA levels.
Takeaway
This study found that a tiny molecule called miR-196a helps control a gene important for early calf development by turning it off.
Methodology
The study used expression analysis, HeLa cell transfections, and microinjection of miR-196a mimics into bovine embryos to assess the regulation of NOBOX.
Statistical Information
P-Value
p<0.05
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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