Unique Protein Profiles in Neutrophils of COPD Patients
Author Information
Author(s): Jeroen D. Langereis, René C. Schweizer, Jan-Willem J. Lammers, Leo Koenderman, Laurien H. Ulfman
Primary Institution: University Medical Center, Utrecht, the Netherlands
Hypothesis
We hypothesized that TNFα/GM-CSF-induced protein profiles can be found in peripheral neutrophils of COPD patients.
Conclusion
Neutrophils from COPD patients have a unique protein profile that does not reflect the protein profiles induced by TNFα or GM-CSF.
Supporting Evidence
- 7 protein spots were significantly regulated between COPD patients and healthy controls.
- In vitro stimulation with TNFα and GM-CSF resulted in 13 and 20 regulated protein spots, respectively.
- The protein profiles of COPD patients did not correspond to those induced by TNFα or GM-CSF.
Takeaway
This study found that the proteins in the blood cells of COPD patients are different from those in healthy people, and the usual signals that cause inflammation don't explain these differences.
Methodology
Fluorescence 2-dimensional difference gel electrophoresis was used to analyze protein profiles in neutrophils from COPD patients and healthy controls.
Potential Biases
Potential confounding due to smoking status of COPD patients.
Limitations
The study lacked an age-matched control group of smokers without COPD, which could confound results.
Participant Demographics
13 COPD patients (11 male, 2 female) and 6 healthy controls (5 male, 1 female), with an average age of 65.5 years for COPD patients and 60.3 years for controls.
Statistical Information
P-Value
p<0.01
Statistical Significance
p<0.01
Digital Object Identifier (DOI)
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