Expression of yeast lipid phosphatase Sac1p is regulated by phosphatidylinositol-4-phosphate
2008
Regulation of Yeast Lipid Phosphatase Sac1p by Phosphatidylinositol-4-Phosphate
publication
Evidence: moderate
Author Information
Author(s): Andreas Knödler, Gerlinde Mayinger, Peter Mayinger
Primary Institution: Oregon Health & Science University
Hypothesis
How is the expression of the SAC1 gene regulated in response to phosphatidylinositol-4-phosphate concentrations?
Conclusion
The expression of Sac1p is regulated by the concentration of its substrate, PI(4)P, ensuring proper maintenance of its levels in the cell.
Supporting Evidence
- The SAC1 gene expression is independent of inositol levels.
- A novel 9-bp motif in the SAC1 promoter is critical for its regulation.
- Sac1p levels correlate with intracellular PI(4)P concentrations.
Takeaway
This study shows that a protein in yeast, Sac1p, is made more when there is more of a specific fat called PI(4)P, helping the cell keep things balanced.
Methodology
The researchers used reporter constructs to measure SAC1 promoter activity and analyzed protein levels through immunoblotting.
Digital Object Identifier (DOI)
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