Rapid Genotyping of Soybean Cultivars Using High Throughput Sequencing
Author Information
Author(s): Varala Kranthi, Swaminathan Kankshita, Li Ying, Hudson Matthew E.
Primary Institution: Department of Crop Sciences, University of Illinois, Urbana-Champaign, Illinois, United States of America
Hypothesis
A rapid method to develop SNP markers that can differentiate specific loci between any two parents in soybean is highly desirable.
Conclusion
The study successfully developed a rapid and reproducible method for generating SNP markers in soybean cultivars.
Supporting Evidence
- The study identified between 4294 to 14550 SNPs between the four accessions and the Williams 82 reference.
- SNPs were filtered to include only high confidence calls based on stringent criteria.
- The method allows for rapid and cost-effective genotyping of soybean accessions.
Takeaway
Scientists found a quick way to identify genetic differences in soybean plants, which helps in breeding better crops.
Methodology
The study used reduced representation libraries (RRLs) of genomic DNA generated by restriction digestion followed by high throughput sequencing.
Potential Biases
Potential bias in SNP calling due to reliance on specific sequencing technologies.
Limitations
The study may not account for all genetic variations within the soybean cultivars due to the sampling method.
Participant Demographics
The study involved five soybean accessions, including both elite and non-elite lines.
Digital Object Identifier (DOI)
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