Circadian Genes in Early Development of Xenopus laevis
Author Information
Author(s): Curran Kristen L., LaRue Silvia, Bronson Brittany, Solis Jessica, Trow Aaron, Sarver Nicole, Zhu Haisun
Primary Institution: University of Wisconsin-Whitewater
Hypothesis
The study investigates the developmental expression of circadian genes to understand the ontogeny of the circadian clock in vertebrates.
Conclusion
Circadian genes are present during early brain and eye development, but rhythmic expression in the eye is not observed until after stage 31 of development.
Supporting Evidence
- All circadian genes analyzed are present during early brain and eye development.
- Rhythmic expression of xBmal1 in the eye is observed only after stage 31.
- Expression patterns of circadian genes differ in various tissues during development.
Takeaway
This study looks at how certain genes that help keep time in our bodies are used during the early growth of frogs, showing they start working in the eyes only when the frogs are older.
Methodology
The study used in situ hybridization and qRT-PCR to analyze the expression of circadian genes in embryos from neurula to late tailbud stages.
Limitations
The study may not account for all potential environmental influences on gene expression.
Statistical Information
P-Value
0.0004
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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