Development of pan-specific antibody against trimethyllysine for protein research
2008
Development of a Specific Antibody for Trimethyllysine in Proteins
publication
Evidence: moderate
Author Information
Author(s): Liang Ziqian, Wong Ronald PC, Li Lin Hong, Jiang Hesheng, Xiao Hao, Li Gang
Primary Institution: Guangxi Medical University
Hypothesis
Can a pan-specific antibody against Nε-trimethyllysine (tMeK) be developed for protein research?
Conclusion
The developed tMeK antibody is effective for isolating and detecting proteins with trimethyllysine residues.
Supporting Evidence
- The antibody specifically reacted to trimethyllysine and not to other methylated forms.
- Western blot analysis confirmed the presence of trimethylated proteins in various tissues.
- The antibody's specificity was validated through competitive inhibition with free tMeK.
Takeaway
Scientists created a special tool that helps find and study proteins with a specific chemical change called trimethyllysine.
Methodology
The antibody was generated using a methylated immunogen and purified through affinity chromatography.
Digital Object Identifier (DOI)
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