Cigarette Smoke Extract and Heme Oxygenase-1 in Brain Cells
Author Information
Author(s): Shih Ruey-Horng, Cheng Shin-Ei, Hsiao Li-Der, Kou Yu Ru, Yang Chuen-Mao
Primary Institution: Chang Gung University
Hypothesis
The study investigates the mechanisms by which cigarette smoke extract modulates heme oxygenase-1 protein expression in cerebral endothelial cells.
Conclusion
Cigarette smoke extract induces heme oxygenase-1 expression through a specific signaling pathway involving PKCδ and NADPH oxidase in brain endothelial cells.
Supporting Evidence
- Cigarette smoke extract significantly induced HO-1 protein expression in brain endothelial cells.
- The induction of HO-1 was mediated through a PKCδ/NADPH oxidase/ROS signaling pathway.
- Inhibitors of PKCδ and NADPH oxidase reduced the expression of HO-1 in response to cigarette smoke extract.
Takeaway
Cigarette smoke can make brain cells produce a protein that can be harmful, and this happens through a specific process in the cells.
Methodology
The study used cultured mouse brain endothelial cells to assess the effects of cigarette smoke extract on heme oxygenase-1 expression and the underlying molecular mechanisms.
Statistical Information
P-Value
p<0.05
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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