Mannan-Aβ28 Conjugate Prevents Amyloid Plaque Deposition but Increases Microhemorrhages in Mice
Author Information
Author(s): Petrushina Irina, Ghochikyan Anahit, Mkrtichyan Mikayel, Mamikonyan Grigor, Movsesyan Nina, Ajdari Rodmehr, Vasilevko Vitaly, Karapetyan Adrine, Lees Andrew, Agadjanyan Michael G, Cribbs David H
Primary Institution: The Institute for Brain Aging and Dementia, University of California Irvine
Hypothesis
Does the mannan-Aβ28 conjugate induce a Th2-type immune response and affect amyloid pathology in Tg2576 mice?
Conclusion
The mannan-Aβ28 conjugate induced a strong anti-Aβ antibody response but also led to increased microhemorrhages in the brains of immunized mice.
Supporting Evidence
- Immunization with mannan-Aβ28 induced long-lasting anti-Aβ antibody responses.
- Vaccination significantly reduced amyloid plaque deposition in the brains of Tg2576 mice.
- Immunized mice showed increased levels of microhemorrhages compared to control groups.
- Anti-Aβ antibodies were predominantly of the IgG1 isotype, indicative of a Th2 immune response.
Takeaway
Researchers created a vaccine that helps mice fight off a brain problem caused by Alzheimer's, but it also caused some bleeding in their brains.
Methodology
Mice were immunized with mannan-Aβ28 conjugate, and immune responses were analyzed using ELISA and immunohistochemistry.
Potential Biases
Potential bias due to the lack of blinding in some assessments.
Limitations
The study did not quantify CAA in the meninges and focused primarily on the neocortex.
Participant Demographics
Tg2576 (APPsw) mice, aged 18-18.5 months.
Statistical Information
P-Value
0.0075 for Aβ42, 0.0103 for Aβ40, 0.0019 for cored plaques
Statistical Significance
p<0.001
Digital Object Identifier (DOI)
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