DNA Double Strand Breaks Occur Independent of AID in Hypermutating Ig Genes
2003
DNA Double Strand Breaks and AID in B Cells
publication
Evidence: moderate
Author Information
Author(s): Linda Bross, Heinz Jacobs
Primary Institution: University of Maastricht
Hypothesis
Does AID function upstream or downstream of DNA lesions in hypermutating Ig genes?
Conclusion
DNA double-strand breaks occur at similar frequencies in both germline and rearranged Ig genes, but somatic hypermutation preferentially targets rearranged genes.
Supporting Evidence
- DNA double-strand breaks are found in both rearranged and germline Ig genes.
- Somatic hypermutation preferentially targets rearranged Ig genes.
- AID deficiency does not significantly affect the frequency or distribution of DNA double-strand breaks.
Takeaway
The study found that DNA breaks happen in both types of Ig genes, but the ones that are rearranged are more likely to get mutated.
Methodology
The study analyzed the Vl locus of AID proficient and deficient B cells for DNA double-strand breaks using PCR and sequencing.
Digital Object Identifier (DOI)
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