Labeling Mesenchymal Stem Cells for MRI with Iron Oxide Nanoparticles
Author Information
Author(s): Schmidtke-Schrezenmeier Gerlinde, Urban Markus, Musyanovych Anna, Mailänder Volker, Rojewski Markus, Fekete Natalie, Menard Cedric, Deak Erika, Tarte Karin, Rasche Volker, Landfester Katharina, Schrezenmeier Hubert
Primary Institution: DRK Blood Service Baden-Württemberg-Hessia, Institute for Clinical Transfusion Medicine and Immunogenetics and Institute of Transfusion Medicine, University of Ulm, Germany
Hypothesis
Can iron oxide-poly(l-lactide) nanoparticles be used to label mesenchymal stromal cells (MSC) for effective magnetic resonance imaging without affecting their functionality?
Conclusion
The study demonstrated that iron oxide-poly(l-lactide) nanoparticles can label MSC effectively for MRI without altering their viability or functionality.
Supporting Evidence
- MSC showed rapid uptake of nanoparticles and long-lasting intracellular persistence.
- Labeling did not influence MSC viability, differentiation, or immunosuppressive properties.
- The nanoparticles exhibited excellent MRI properties in agarose phantoms and in vivo.
- MSC functionality was preserved after labeling with iron oxide-poly(l-lactide) nanoparticles.
Takeaway
Scientists found a way to mark special cells called MSC with tiny particles so they can be seen in pictures without hurting the cells.
Methodology
The study used flow cytometry, transmission electron microscopy, confocal laser scanning microscopy, and various assays to evaluate the effects of the nanoparticles on MSC.
Limitations
The study did not assess long-term effects beyond the initial follow-up period and focused primarily on specific nanoparticle types.
Participant Demographics
Human bone marrow-derived MSC were used in the study.
Digital Object Identifier (DOI)
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