Crystal Structure of a Protein from Pseudomonas aeruginosa
Author Information
Author(s): Clarke Teresa E, Romanov Vladimir, Chirgadze Yuri N, Klomsiri Chananat, Kisselman Gera, Wu-Brown Jean, Poole Leslie B, Pai Emil F, Chirgadze Nickolay Y
Primary Institution: Campbell Cancer Research Institute, Ontario Cancer Institute, Princess Margaret Hospital, University Health Network, Toronto, Ontario, Canada
Hypothesis
What is the structure and potential function of the hypothetical protein PA0269 from Pseudomonas aeruginosa?
Conclusion
The protein PA0269 is likely a weak hydroperoxidase with peroxidase-related activity.
Supporting Evidence
- The protein has an all-helical fold with a chain topology similar to a known AhpD from Mycobacterium tuberculosis.
- A conserved two α-helical motif responsible for function is present in both proteins.
- PA0269 exhibits a weak ability to reduce H2O2 as tested using a ferrous oxidation-xylenol orange (FOX) assay.
Takeaway
Scientists studied a protein from bacteria that helps protect against harmful substances, and they found it might work like a similar protein in another bacteria.
Methodology
The crystal structure of PA0269 was determined using X-ray crystallography.
Limitations
The binding sites for substrates or inhibitors are not yet known.
Digital Object Identifier (DOI)
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