Smac/DIABLO enhances the therapeutic potential of chemotherapeutic drugs and irradiation, and sensitizes TRAIL-resistant breast cancer cells
2008
Smac/DIABLO Enhances Cancer Treatment
publication
Evidence: high
Author Information
Author(s): Fandy Tamer E, Shankar Sharmila, Srivastava Rakesh K
Primary Institution: The Sidney Kimmel Comprehensive Cancer Center at Johns Hopkins
Hypothesis
Can Smac/DIABLO enhance the effectiveness of chemotherapeutic drugs and irradiation in breast cancer cells?
Conclusion
Smac/DIABLO can enhance the effectiveness of chemotherapeutic drugs and irradiation, and sensitizes TRAIL-resistant breast cancer cells to apoptosis.
Supporting Evidence
- Smac/DIABLO peptide enhances apoptosis induced by various chemotherapeutic drugs.
- Overexpression of Smac/DIABLO sensitizes TRAIL-resistant breast cancer cells.
- Smac/DIABLO increases caspase-3 activity, promoting apoptosis.
- Combination of Smac/DIABLO with TRAIL leads to increased apoptosis in resistant cells.
Takeaway
Smac/DIABLO helps cancer treatments work better by making cancer cells more likely to die when treated with drugs or radiation.
Methodology
Breast cancer cells were treated with Smac/DIABLO gene or peptide, and cell viability and apoptosis were measured using XTT assay and DAPI staining.
Statistical Information
P-Value
p<0.05
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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