Correlative and Dynamic Imaging of the Hatching Biology of Schistosoma japonicum from Eggs Prepared by High Pressure Freezing
2008

Hatching Biology of Schistosoma japonicum Eggs

publication Evidence: high

Author Information

Author(s): Malcolm K. Jones, Sze How Bong, Kathryn M. Green, Philadelphia Holmes, Mary Duke, Alex Loukas, Donald P. McManus

Primary Institution: Queensland Institute of Medical Research

Hypothesis

What are the mechanisms of activation and hatching in Schistosoma japonicum eggs?

Conclusion

The hatching of Schistosoma japonicum eggs involves explosive liberation of the larva while still encapsulated within its sub-shell envelopes.

Supporting Evidence

  • The hatching behavior was recorded by video microscopy.
  • High pressure freezing was used to preserve the ultrastructure of the eggs.
  • The study provided insights into the dynamic activity of the eggs and the biology of schistosomes within the host.

Takeaway

This study looks at how the eggs of a parasite called Schistosoma japonicum hatch. The eggs burst open to let out tiny baby worms, and this happens in a very special way.

Methodology

The study used correlative light and electron microscopy, video microscopy, and high pressure freezing to observe the hatching process.

Limitations

The study may not account for all environmental factors affecting hatching, and the use of praziquantel may have influenced the results.

Digital Object Identifier (DOI)

10.1371/journal.pntd.0000334

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