Paper-Based Aptasensor Assay for Detection of Food Adulterant Sildenafil
2024
Detecting Sildenafil in Food with a Paper Test
Sample size: 49
publication
Evidence: moderate
Author Information
Author(s): Kavruk Murat, Ozalp Veli Cengiz
Primary Institution: Istanbul Aydin University
Hypothesis
Can a lateral flow strip test using aptamers effectively detect sildenafil in food products?
Conclusion
The study successfully developed a lateral flow assay that can detect sildenafil in food samples with a detection limit of 68.2 nM.
Supporting Evidence
- Sildenafil was found in 65% of tested food products in Croatia and 56.3% in Saudi Arabia.
- The detection limit for the developed strips was 68.2 nM in spiked food samples.
- The method simplifies the lateral flow assay for small molecules and provides a tool for signal amplification.
- A linear relationship between TMB release and sildenafil concentration was obtained with a high correlation (R2 = 0.98).
- The study highlights the need for innovation in lateral flow biosensors for small molecule detection.
Takeaway
This study created a simple paper test to check if food has sildenafil, a drug that shouldn't be there.
Methodology
The study used aptamers selected through graphene oxide SELEX to create a lateral flow strip test for detecting sildenafil in food.
Limitations
The sensitivity of the method is lower compared to traditional laboratory techniques like HPLC.
Digital Object Identifier (DOI)
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