Evaluation of 15 Functional Candidate Genes for Association with Chronic Otitis Media with Effusion and/or Recurrent Otitis Media (COME/ROM)
2011

Genetic Factors in Chronic Otitis Media with Effusion and Recurrent Otitis Media

Sample size: 618 publication Evidence: moderate

Author Information

Author(s): Sale Michèle M., Chen Wei-Min, Weeks Daniel E., Mychaleckyj Josyf C., Hou Xuanlin, Marion Miranda, Segade Fernando, Casselbrant Margaretha L., Mandel Ellen M., Ferrell Robert E., Rich Stephen S., Daly Kathleen A.

Primary Institution: University of Virginia

Hypothesis

DNA sequence variants in genes involved in the innate immune response and secondary response to infection may confer susceptibility to chronic otitis media with effusion and/or recurrent otitis media (COME/ROM).

Conclusion

Common variants in the selected candidate genes do not appear to make a major contribution to COME/ROM susceptibility.

Supporting Evidence

  • SNP rs2735733 in the MUC5AC gene region exhibited nominal evidence for association with COME/ROM (P=0.002).
  • Two additional SNPs from this region had P values<0.05.
  • Other variants exhibiting associations with COME/ROM at P<0.05 included the SCN1B SNP rs8100085 (P=0.013), SFTPD SNP rs1051246 (P=0.039) and TLR4 SNP rs2770146 (P=0.038).
  • Age at exam was the factor most significantly associated with COME/ROM.

Takeaway

The study looked at genes that might make kids more likely to get ear infections, but found that these genes don't really seem to matter much.

Methodology

The study evaluated single nucleotide polymorphisms (SNPs) in 15 candidate genes in 142 families using the Generalized Disequilibrium Test (GDT).

Potential Biases

Genotyping inaccuracies could potentially lead to false negative results.

Limitations

The study included a small sample of non-European families, limiting conclusions about the generalizability of the findings.

Participant Demographics

The population included 618 individuals from 142 families, primarily non-Hispanic and of European ancestry.

Statistical Information

P-Value

0.002

Statistical Significance

p<0.05

Digital Object Identifier (DOI)

10.1371/journal.pone.0022297

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