PCNA interacts with Prox1 and represses its transcriptional activity
2008
PCNA and Prox1 Interaction in Lens Development
publication
Evidence: moderate
Author Information
Author(s): Chen Xiaoren, Patel Tapan P., Simirskii Vladimir I., Duncan Melinda K.
Primary Institution: Department of Biological Sciences, University of Delaware, Newark, DE
Hypothesis
This paper seeks to better understand the role of protein–protein interactions in the functions of Prox1.
Conclusion
Prox1-PCNA interactions may prevent the activation of βB1-crystallin expression in the lens epithelium.
Supporting Evidence
- Prox1 is a transcription factor necessary for the development of diverse organs including the lens.
- PCNA was identified as a Prox1 interacting protein through a yeast two-hybrid screen.
- Prox1 interactions with PCNA require the PCNA interacting protein motif located in the Prospero domain of Prox1.
- PCNA repressed Prox1 mediated activation of the chicken βB1-crystallin promoter in transfection assays.
Takeaway
Prox1 is a protein that helps develop the lens in the eye, and it works with another protein called PCNA to control how lens cells grow.
Methodology
Yeast two-hybrid screen, computer modeling, cotransfection analysis, and confocal immunolocalization were used to investigate Prox1 interactions.
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