Study of Immune Genes in Wild Silkmoth
Author Information
Author(s): Gandhe Archana S, Arunkumar KP, John Serene H, Nagaraju J
Primary Institution: Centre for DNA Fingerprinting and Diagnostics, Hyderabad, India
Hypothesis
What immune-related genes are present in the transcriptome of the wild silkmoth Antheraea mylitta when challenged with bacteria?
Conclusion
The study identified many potential immune-related genes in A. mylitta, some of which are similar to vertebrate genes and others are novel.
Supporting Evidence
- The study identified 719 unique ESTs from 1412 sequences.
- 31% of the transcriptome showed similarity to known or putative immune proteins.
- The majority of immune-related genes were more induced by E. coli than by Micrococcus luteus.
Takeaway
Scientists looked at the genes in a silkmoth's body to see how it fights off germs, and they found many important genes that help with this.
Methodology
Constructed and analyzed an immune transcriptome from fat body tissues of E. coli-challenged A. mylitta larvae.
Limitations
The EST library was not normalized, which may have led to missing less abundant transcripts.
Participant Demographics
5th instar larvae of Antheraea mylitta.
Digital Object Identifier (DOI)
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