Chemopotentiation of CCNU metabolites by misonidazole
1985

Enhancing the Effectiveness of CCNU with Misonidazole

publication Evidence: moderate

Author Information

Author(s): R.T. Mulcahy

Primary Institution: University of Rochester

Hypothesis

Can Misonidazole enhance the cytotoxicity of CCNU metabolites?

Conclusion

Misonidazole significantly enhances the cytotoxicity of CCNU metabolites in vitro.

Supporting Evidence

  • Misonidazole enhances the cytotoxicity of CCNU metabolites.
  • Cis-4-OH CCNU is more toxic than CCNU.
  • Cis-2-OH CCNU is less effective than CCNU.
  • All four isomers' cytotoxicity is significantly enhanced with Misonidazole.

Takeaway

This study shows that a drug called Misonidazole can make another drug, CCNU, work better against cancer cells.

Methodology

EMT-6/Ro tumor cells were treated in vitro with Misonidazole and various hydroxylated metabolites of CCNU under hypoxic conditions.

Statistical Information

P-Value

p<0.01

Confidence Interval

1.2-1.4, 1.8-2.2, 1.7-1.9, 1.6-1.9

Statistical Significance

p<0.05

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