Correction on Parp-1 and Gene Expression in Cells
Author Information
Author(s): Ogino Hideki, Nozaki Tadashige, Gunji Akemi, Maeda Miho, Suzuki Hiroshi, Ohta Tsutomu, Murakami Yasufumi, Nakagama Hitoshi, Sugimura Takashi, Masutani Mitsuko
Primary Institution: National Cancer Center Research Institute
Hypothesis
How does the loss of Parp-1 affect gene expression profiles in ES cells and liver cells?
Conclusion
The correction revealed that 3.6% of genes showed altered expression in Parp-1-/- ES cells, with 2.5% down-regulated and 1.1% up-regulated.
Supporting Evidence
- 3.6% of genes showed altered expression in Parp-1-/- ES cells.
- 2.5% of the genes were down-regulated by 2-fold or greater.
- 1.1% of the genes were up-regulated by 2-fold or greater.
Takeaway
When a specific gene called Parp-1 is missing, it changes how many genes work in certain cells, with some genes being turned off and others turned on.
Methodology
Microarray data analysis was performed using GeneSpring software, comparing gene expression between Parp-1+/+ and Parp-1-/- ES cells.
Limitations
The initial analysis included incorrect data due to careless inclusion of replicates.
Statistical Information
P-Value
p < 0.05
Statistical Significance
p < 0.05
Digital Object Identifier (DOI)
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