Extracting Cytokines and MMPs from Schirmer Strips
Author Information
Author(s): VanDerMeid Karl R., Su Stephanie P., Krenzer Kathleen L., Ward Keith W., Zhang Jin-Zhong
Primary Institution: Bausch + Lomb, Inc.
Hypothesis
Can cytokines and matrix metalloproteinases be effectively extracted from Schirmer strips for analysis?
Conclusion
The study found that multiple cytokines and MMPs can be detected in tear samples collected using Schirmer strips, which may aid in evaluating dry eye disease.
Supporting Evidence
- All cytokines and MMPs tested were detected in at least 50% of the samples.
- Recoveries from extracted Schirmer strips were greater than 60% for 8 of the 10 cytokines.
- The method allows for the evaluation of both the quantity and quality of tears.
Takeaway
Researchers figured out how to collect and measure important proteins from tears using a common eye test strip, which can help understand dry eye problems better.
Methodology
Tears were collected from 5 subjects using Schirmer strips, and cytokines and MMPs were extracted and analyzed using Luminex technology.
Potential Biases
Potential bias due to small sample size and the possibility of tear reflection affecting cytokine concentrations.
Limitations
The sample size was small, and some cytokines were below the limit of detection in certain samples.
Participant Demographics
Five healthy volunteers aged 25 to 54, with varying Ocular Surface Disease Index scores.
Statistical Information
P-Value
p<0.05
Statistical Significance
p<0.05
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