Promoter region methylation does not account for the frequent loss of expression of the Fas gene in colorectal carcinoma
2000
Fas Gene Expression Loss in Colorectal Cancer
Sample size: 53
publication
Author Information
Author(s): Butler L M, Dobrovic A, Bianco T, Cowled P A
Primary Institution: The University of Adelaide and The Queen Elizabeth Hospital
Hypothesis
Does methylation of the Fas promoter inhibit gene expression in colorectal carcinomas?
Conclusion
Methylation is not primarily responsible for the loss of expression of the Fas gene in colorectal tumors.
Supporting Evidence
- The Fas gene is often not expressed in colorectal cancer.
- No methylation was detected in the Fas promoter region in any of the examined specimens.
Takeaway
The study looked at whether a specific change in DNA could stop a gene from working in colon cancer, and found that it doesn't.
Methodology
Examined 47 colorectal adenoma and carcinoma specimens and 6 normal colonic mucosa samples for methylation using Southern blotting.
Digital Object Identifier (DOI)
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