Cell Size at S Phase Initiation in Yeast
Author Information
Author(s): Barberis Matteo, Klipp Edda, Vanoni Marco, Alberghina Lilia
Primary Institution: Department of Biotechnology and Biosciences, University of Milano-Bicocca, Milan, Italy
Hypothesis
The critical cell size required for the G1/S transition in budding yeast is an emergent property of the G1 to S network that depends on growth rate.
Conclusion
The study presents a mathematical model showing that the critical cell size for DNA replication in yeast is influenced by growth conditions and is an emergent property of the regulatory network.
Supporting Evidence
- The model accounts for the localization of proteins in the nucleus and cytoplasm.
- Sensitivity analysis indicates that the critical cell size is influenced by the growth rate.
- The model predicts that the binding affinity of Sic1 to Cdk1-Clb5,6 is modulated by growth conditions.
Takeaway
Yeast cells need to grow to a certain size before they can start making new DNA, and this size changes depending on how fast they are growing.
Methodology
A mathematical model was developed using ordinary differential equations to simulate the G1 to S transition in yeast under different growth conditions.
Limitations
The model does not include a description of other cell cycle events beyond the G1/S transition.
Statistical Information
P-Value
p<0.05
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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