High Throughput Antibody Generation Using Phage Display
Author Information
Author(s): Darren J Schofield, Anthony R Pope, Veronica Clementel, Jenny Buckell, Susan DJ Chapple, Kay F Clarke, Jennie S Conquer, Anna M Crofts, Sandra RE Crowther, Michael R Dyson, Gillian Flack, Gareth J Griffin, Yvette Hooks, William J Howat, Anja Kolb-Kokocinski, Susan Kunze, Cecile D Martin, Gareth L Maslen, Joanne N Mitchell, Maureen O'Sullivan, Rajika L Perera, Wendy Roake, S Paul Shadbolt, Karen J Vincent, Anthony Warford, Wendy E Wilson, Jane Xie, Joyce L Young, John McCafferty
Primary Institution: Wellcome Trust Sanger Institute
Hypothesis
Can a phage display library be effectively used to generate and characterize a large number of antibodies?
Conclusion
The study successfully created a high-quality phage display library containing over 10 billion human antibodies, leading to the generation of over 7,200 unique recombinant antibodies.
Supporting Evidence
- The library contained over 10 billion human antibodies.
- Over 38,000 recombinant antibodies were selected and screened.
- 4,400 unique clones were characterized further.
- 37% of antibodies showed positive staining in tissue microarrays.
Takeaway
Scientists made a huge library of antibodies to help study proteins better, and they found many unique antibodies that can be used for research.
Methodology
The study involved constructing a phage display library, selecting and screening over 38,000 recombinant antibodies against 292 antigens, and characterizing 4,400 unique clones.
Limitations
The success rate of antibody generation varied based on the source of antigens, with mammalian-produced antigens performing better than those produced in bacteria.
Participant Demographics
Antibodies were generated from B lymphocytes collected from 43 human donors.
Digital Object Identifier (DOI)
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