Emulsion PCR: A High Efficient Way of PCR Amplification of Random DNA Libraries in Aptamer Selection
Author Information
Author(s): Shao Keke, Ding Weifeng, Wang Feng, Li Haiquan, Ma Da, Wang Huimin
Primary Institution: Clinical Laboratory, Yancheng City NO.1 People's Hospital, Yancheng, China; Laboratory Center, Affiliated Hospital of Nantong University, Nantong, China
Hypothesis
Can emulsion PCR reduce by-product formation and increase product formation in aptamer selection?
Conclusion
Emulsion PCR significantly improves the efficiency of SELEX by reducing by-product formation and increasing product yield.
Supporting Evidence
- Emulsion PCR reduced by-product formation to an undetectable level.
- Product formation increased significantly with emulsion PCR compared to conventional PCR.
- The optimal ratio of template to compartment was found to be crucial for minimizing by-products.
- Concentration of Taq DNA polymerase significantly impacted product formation efficiency.
Takeaway
This study shows that using emulsion PCR can help scientists make more of the good stuff they want while making less of the unwanted stuff when selecting DNA sequences.
Methodology
The study compared conventional PCR and emulsion PCR for amplifying random DNA libraries, analyzing product and by-product formation through various cycles and conditions.
Limitations
The study primarily focuses on the efficiency of emulsion PCR without extensive exploration of other potential factors affecting aptamer selection.
Digital Object Identifier (DOI)
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