How Acetylation Activates Abl's Apoptotic Function
Author Information
Author(s): Jiang Zhihua, Kamath Ravindra, Jin Shunquian, Balasubramani Manimalha, Pandita Tej K, Rajasekaran Baskaran
Primary Institution: University Drive Pittsburgh, PA 15240, Pittsburgh PA 15261, USA
Hypothesis
Does acetylation of Abl enhance its apoptotic activity in response to DNA damage?
Conclusion
Acetylation of Abl at K921, mediated by Tip60, promotes transcription-independent apoptosis following DNA damage.
Supporting Evidence
- Acetylation of Abl at K921 is induced by DNA damage.
- Cells expressing the non-acetylatable K921R mutant showed reduced apoptosis.
- Tip60 was identified as the acetylase responsible for modifying Abl.
Takeaway
When cells are damaged, a protein called Abl gets a special tag (acetylation) that helps it make cells die when they should. This is important for keeping our cells healthy.
Methodology
The study used mass spectrometry and immunoprecipitation to analyze acetylation of Abl in response to DNA damage.
Statistical Information
P-Value
p<0.01
Statistical Significance
p<0.01
Digital Object Identifier (DOI)
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