Dermal Macrophages Resist Dengue Virus Growth
Author Information
Author(s): Kwan Wing-Hong, Navarro-Sanchez Erika, Dumortier Hélène, Decossas Marion, Vachon Hortense, dos Santos Flavia Barreto, Fridman Hervé W., Rey Félix A., Harris Eva, Despres Philippe, Mueller Christopher G.
Primary Institution: CNRS, Laboratory of Therapeutic Immunology and Chemistry, IBMC, Université Louis Pasteur, Strasbourg, France
Hypothesis
Are dermal macrophages resistant to dengue virus infection?
Conclusion
Dermal macrophages can internalize dengue virus but do not produce progeny virus due to the accumulation of the virus in poorly-acidified phagosomes.
Supporting Evidence
- Dermal macrophages express CD209 and can bind dengue virus envelope protein.
- Monocyte-derived dermal macrophages were resistant to dengue virus replication.
- Dengue virus was internalized but not produced in dermal macrophages.
- Accumulation of the virus in poorly-acidified phagosomes prevented infection.
Takeaway
The study found that special immune cells in the skin called dermal macrophages can catch the dengue virus but don't let it grow, which helps protect the body from getting sick.
Methodology
The researchers generated dermal-type macrophages from monocytes and tested their ability to bind and internalize dengue virus.
Potential Biases
Potential bias in the selection of healthy donors for skin samples.
Limitations
The study did not explore the long-term effects of dengue virus on dermal macrophages or the potential for other pathogens to exploit these cells.
Participant Demographics
Healthy adult human donors undergoing plastic surgery.
Statistical Information
P-Value
p<0.05
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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