Fasudil Protects Zebrafish from Cisplatin-Induced Hearing Loss
Author Information
Author(s): Lim Kang Hyeon, Park Saemi, Han Eunjung, Baek Hyun woo, Hyun Kyungtae, Hong Sumin, Kim Hwee-Jin, Lee Yunkyoung, Rah Yoon Chan, Choi June, Matsuzawa Atsushi
Primary Institution: Korea University College of Medicine, Ansan Hospital
Hypothesis
Fasudil, a ROCK inhibitor, can protect against cisplatin-induced ototoxicity in zebrafish.
Conclusion
Fasudil effectively mitigates hair cell loss and apoptosis caused by cisplatin in zebrafish.
Supporting Evidence
- Cisplatin significantly reduced hair cell counts in zebrafish.
- Fasudil co-treatment preserved hair cell counts compared to cisplatin alone.
- Fasudil reduced apoptosis and ROS levels in hair cells.
- Rheotaxis behavior was improved with fasudil treatment.
Takeaway
Cisplatin can hurt hearing by damaging hair cells, but fasudil can help protect those cells.
Methodology
Zebrafish larvae were treated with cisplatin and fasudil, and various assays were conducted to assess hair cell counts, apoptosis, and behavior.
Limitations
The study did not confirm fasudil's absorption into hair cells or the activation of the ROCK pathway by cisplatin.
Participant Demographics
Zebrafish larvae, specifically the (Brn3c:EGFP)s356t transgenic line.
Statistical Information
P-Value
p<0.001
Statistical Significance
p<0.001
Digital Object Identifier (DOI)
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