Synthetic Emmprin Peptides Stimulate MMP-2 Production
Author Information
Author(s): Kawakami Takehito, Sameshima Tetsuro, Hojo Hironobu, Koga Kaori, Nakahara Yoshiaki, Toole Bryan P, Suzumiya Junji, Okada Yasunori, Iwasaki Akinori, Nabeshima Kazuki
Primary Institution: Fukuoka University School of Medicine
Hypothesis
Can synthetic emmprin ECI peptides with chitobiose substitution mimic the activity of natural emmprin in stimulating MMP-2 production by fibroblasts?
Conclusion
Synthetic emmprin ECI peptides can stimulate MMP-2 production by fibroblasts when substituted with chitobiose.
Supporting Evidence
- ECI carrying a chitobiose unit stimulated MMP-2 production by fibroblasts.
- Unsubstituted ECI had no stimulatory effect on MMP-2 production.
- ECI-(GlcNAc)2 showed a dose-dependent increase in MMP-2 production.
- Longer chitobiose units stimulated MMP-2 production but less effectively than ECI-(GlcNAc)2.
- Anti-emmprin blocking antibody inhibited ECI-(GlcNAc)2-induced MMP-2 production.
Takeaway
The study found that a special sugar added to a protein can help it tell cells to make a substance that helps in tissue repair and cancer spread.
Methodology
Synthetic ECI peptides were tested on fibroblasts to see if they stimulated MMP-2 production.
Statistical Information
P-Value
p<0.01
Statistical Significance
p<0.01
Digital Object Identifier (DOI)
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