Proteome-level display of bladder cancer cell phenotype modulation
Author Information
Author(s): Robert E. Hurst, Kimberly D. Kyker, Mikhail G. Dozmorov, Nobuaki Takemori, Anil Singh, Hiroyuki Matsumoto, Ricardo Saban, Edna Betgovargez, Michael H. Simonian
Primary Institution: Oklahoma University Health Sciences Centre
Hypothesis
The extracellular matrix can modulate the phenotype of bladder cancer cells.
Conclusion
The study demonstrates that the MYC pathway is crucial to the malignant phenotype of bladder cancer cells.
Supporting Evidence
- Cells grown on Matrigel expressed unique proteins related to the MYC pathway.
- Proteins identified showed significant post-translational modifications.
- Two-dimensional chromatography provided higher sensitivity than traditional gel methods.
Takeaway
This study shows that the environment where bladder cancer cells grow can change how they behave, and some proteins are important for this change.
Methodology
Bladder cancer cells were grown on different extracellular matrix preparations and analyzed using 2-dimensional chromatography and mass spectrometry.
Limitations
The study may not account for all variables affecting protein expression in different environments.
Statistical Information
P-Value
p<0.05
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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