Conserved cis-elements bind a protein complex that regulates Drosophila ras2/rop bidirectional expression
1994
Regulation of Drosophila ras2/rop Gene Expression
publication
Evidence: moderate
Author Information
Author(s): K. Lightfoot, L. Maltby, R. Duarte, R. Veale, O. Segev
Primary Institution: Department of Zoology, University of the Witwatersrand
Hypothesis
The Drosophila ras2 promoter region exhibits bidirectional activity and is regulated by a protein complex.
Conclusion
The study shows that the ras2/rop bidirectional promoter supports the transcription of both genes through a single protein complex.
Supporting Evidence
- The Drosophila ras2 promoter region shares a CACCC box and an AP-1-like sequence with the human c-Ha-ras1 promoter.
- A 14 bp promoter fragment containing a CACCC element interacts with a specific transcription factor.
- Binding sites for protein factors A and B are essential for 95% expression of both genes flanking the promoter.
Takeaway
This research found that two genes in fruit flies work together and are controlled by the same set of proteins, which helps them turn on and off.
Methodology
The study involved plasmid constructions, DNA transfection, CAT assays, and gel mobility-shift assays to analyze protein-DNA interactions.
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