Comparison of DNA Delivery Methods to the Skin
Author Information
Author(s): Loree C. Heller, Mark J. Jaroszeski, Domenico Coppola, Richard Heller
Primary Institution: University of South Florida
Hypothesis
Is electroporation more effective than liposome-mediated delivery for plasmid DNA to the skin?
Conclusion
Electroporation enhances reporter plasmid delivery to the skin more than the liposome conjugation method, but multiple deliveries do not significantly increase expression duration.
Supporting Evidence
- Electroporation significantly increased reporter expression nearly 20 fold compared to liposome delivery.
- Multiple deliveries did not significantly increase the duration of transgene expression.
- Tissue damage was observed after electroporation, particularly after multiple treatments.
Takeaway
Using electricity can help deliver DNA into the skin better than using tiny bubbles, but doing it many times doesn't make it work longer.
Methodology
The study tested plasmid delivery using electroporation and liposome complexed delivery in rodent skin, measuring expression levels and tissue damage.
Potential Biases
Potential bias due to the authors' involvement in related patents and technologies.
Limitations
The study only tested specific conditions and may not generalize to all skin types or delivery methods.
Participant Demographics
Female BALB/c mice and male Sprague Dawley rats, aged 6-7 weeks and weighing 200-250 grams, respectively.
Statistical Information
P-Value
p<0.001
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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